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pcmv flag s127a yap  (Addgene inc)


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    Structured Review

    Addgene inc pcmv flag s127a yap
    Pcmv Flag S127a Yap, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv+yap/pCMV-flag+S127A+YAP+(Plasmid+%2327370)/bio_rxiv__2025__10__16__682743-68-14-15
    Average 93 stars, based on 36 article reviews
    pcmv flag s127a yap - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Transfection:

    Article Title: AAV-mediated YAP expression in cardiac fibroblasts promotes inflammation and increases fibrosis.
    Article Snippet: The 1.5 kb sequence immediately proximal to the first exon of the rat Ccl2 gene was cloned into the XhoI and HindIII sites of the pGL3 Basic luciferase reporter plasmid (Promega). .. Cells were co-transfected with pCMV-eGFP (Addgene #13031) or pCMV-YAP(S127A) (Addgene #27370), and treated with verteporfin (Sigma #SML0534) or DMSO vehicle for 24 h. Following transfection and treatments, cells were lysed with Passive Lysis Buffer (Promega), and luciferase activity was measured using the luciferase assay system (Promega) with an OPTOCOMP I luminometer (MGM Instruments). ..

    Article Title: AAV-mediated YAP expression in cardiac fibroblasts promotes inflammation and increases fibrosis
    Article Snippet: The 1.5 kb sequence immediately proximal to the first exon of the rat Ccl2 gene was cloned into the XhoI and HindIII sites of the pGL3 Basic luciferase reporter plasmid (Promega). .. Cells were co-transfected with pCMV-eGFP (Addgene #13031) or pCMV-YAP(S127A) (Addgene #27370), and treated with verteporfin (Sigma #SML0534) or DMSO vehicle for 24 h. Following transfection and treatments, cells were lysed with Passive Lysis Buffer (Promega), and luciferase activity was measured using the luciferase assay system (Promega) with an OPTOCOMP I luminometer (MGM Instruments). ..

    Lysis:

    Article Title: AAV-mediated YAP expression in cardiac fibroblasts promotes inflammation and increases fibrosis.
    Article Snippet: The 1.5 kb sequence immediately proximal to the first exon of the rat Ccl2 gene was cloned into the XhoI and HindIII sites of the pGL3 Basic luciferase reporter plasmid (Promega). .. Cells were co-transfected with pCMV-eGFP (Addgene #13031) or pCMV-YAP(S127A) (Addgene #27370), and treated with verteporfin (Sigma #SML0534) or DMSO vehicle for 24 h. Following transfection and treatments, cells were lysed with Passive Lysis Buffer (Promega), and luciferase activity was measured using the luciferase assay system (Promega) with an OPTOCOMP I luminometer (MGM Instruments). ..

    Article Title: AAV-mediated YAP expression in cardiac fibroblasts promotes inflammation and increases fibrosis
    Article Snippet: The 1.5 kb sequence immediately proximal to the first exon of the rat Ccl2 gene was cloned into the XhoI and HindIII sites of the pGL3 Basic luciferase reporter plasmid (Promega). .. Cells were co-transfected with pCMV-eGFP (Addgene #13031) or pCMV-YAP(S127A) (Addgene #27370), and treated with verteporfin (Sigma #SML0534) or DMSO vehicle for 24 h. Following transfection and treatments, cells were lysed with Passive Lysis Buffer (Promega), and luciferase activity was measured using the luciferase assay system (Promega) with an OPTOCOMP I luminometer (MGM Instruments). ..

    Luciferase:

    Article Title: AAV-mediated YAP expression in cardiac fibroblasts promotes inflammation and increases fibrosis.
    Article Snippet: The 1.5 kb sequence immediately proximal to the first exon of the rat Ccl2 gene was cloned into the XhoI and HindIII sites of the pGL3 Basic luciferase reporter plasmid (Promega). .. Cells were co-transfected with pCMV-eGFP (Addgene #13031) or pCMV-YAP(S127A) (Addgene #27370), and treated with verteporfin (Sigma #SML0534) or DMSO vehicle for 24 h. Following transfection and treatments, cells were lysed with Passive Lysis Buffer (Promega), and luciferase activity was measured using the luciferase assay system (Promega) with an OPTOCOMP I luminometer (MGM Instruments). ..

    Article Title: AAV-mediated YAP expression in cardiac fibroblasts promotes inflammation and increases fibrosis
    Article Snippet: The 1.5 kb sequence immediately proximal to the first exon of the rat Ccl2 gene was cloned into the XhoI and HindIII sites of the pGL3 Basic luciferase reporter plasmid (Promega). .. Cells were co-transfected with pCMV-eGFP (Addgene #13031) or pCMV-YAP(S127A) (Addgene #27370), and treated with verteporfin (Sigma #SML0534) or DMSO vehicle for 24 h. Following transfection and treatments, cells were lysed with Passive Lysis Buffer (Promega), and luciferase activity was measured using the luciferase assay system (Promega) with an OPTOCOMP I luminometer (MGM Instruments). ..

    Activity Assay:

    Article Title: AAV-mediated YAP expression in cardiac fibroblasts promotes inflammation and increases fibrosis.
    Article Snippet: The 1.5 kb sequence immediately proximal to the first exon of the rat Ccl2 gene was cloned into the XhoI and HindIII sites of the pGL3 Basic luciferase reporter plasmid (Promega). .. Cells were co-transfected with pCMV-eGFP (Addgene #13031) or pCMV-YAP(S127A) (Addgene #27370), and treated with verteporfin (Sigma #SML0534) or DMSO vehicle for 24 h. Following transfection and treatments, cells were lysed with Passive Lysis Buffer (Promega), and luciferase activity was measured using the luciferase assay system (Promega) with an OPTOCOMP I luminometer (MGM Instruments). ..

    Article Title: AAV-mediated YAP expression in cardiac fibroblasts promotes inflammation and increases fibrosis
    Article Snippet: The 1.5 kb sequence immediately proximal to the first exon of the rat Ccl2 gene was cloned into the XhoI and HindIII sites of the pGL3 Basic luciferase reporter plasmid (Promega). .. Cells were co-transfected with pCMV-eGFP (Addgene #13031) or pCMV-YAP(S127A) (Addgene #27370), and treated with verteporfin (Sigma #SML0534) or DMSO vehicle for 24 h. Following transfection and treatments, cells were lysed with Passive Lysis Buffer (Promega), and luciferase activity was measured using the luciferase assay system (Promega) with an OPTOCOMP I luminometer (MGM Instruments). ..



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